<?xml version="1.0" encoding="US-ASCII" standalone="no"?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 5.25b -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: https://www.novaworks.com -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:xbrli="http://www.xbrl.org/2003/instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
    <link:roleRef roleURI="http://imstii/role/RiskReturn" xlink:href="imstii-20250228.xsd#RiskReturn" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/ShareholderFeesData" xlink:href="imstii-20250228.xsd#ShareholderFeesData" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/OperatingExpensesData" xlink:href="imstii-20250228.xsd#OperatingExpensesData" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/ExpenseExample" xlink:href="imstii-20250228.xsd#ExpenseExample" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/ExpenseExampleNoRedemption" xlink:href="imstii-20250228.xsd#ExpenseExampleNoRedemption" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/BarChartData" xlink:href="imstii-20250228.xsd#BarChartData" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/PerformanceTableData" xlink:href="imstii-20250228.xsd#PerformanceTableData" xlink:type="simple" />
    <link:roleRef roleURI="http://imstii/role/RiskReturnDetail" xlink:href="imstii-20250228.xsd#RiskReturnDetail" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/RiskReturnDetailData" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#RiskReturnDetailData" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/Prospectus" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#ProspectusOnly" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/Series" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#SeriesOnly" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/Class" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#ClassOnly" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/PerformanceMeasure" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#PerformanceMeasureOnly" xlink:type="simple" />
    <link:roleRef roleURI="http://xbrl.sec.gov/rr/role/Risk" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#RiskOnly" xlink:type="simple" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#notAll" arcroleURI="http://xbrl.org/int/dim/arcrole/notAll" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" />
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/RiskReturn" xlink:title="00000003 - Document - Risk/Return Summary {Unlabeled}">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskAxis" xlink:label="loc_rrRiskAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrRiskAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AllRisksMember" xlink:label="loc_rrAllRisksMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrRiskAxis" xlink:to="loc_rrAllRisksMember" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AllRisksMember" xlink:label="loc_rrAllRisksMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrRiskAxis" xlink:to="loc_rrAllRisksMember_10" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskLoseMoneyMember" xlink:label="loc_rrRiskLoseMoneyMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_rrRiskLoseMoneyMember_10" xlink:type="arc" order="12" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_MarketRiskMember" xlink:label="loc_imstiiMarketRiskMember_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiMarketRiskMember_20" xlink:type="arc" order="22" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_HighYieldJunkBondRiskMember" xlink:label="loc_imstiiHighYieldJunkBondRiskMember_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiHighYieldJunkBondRiskMember_30" xlink:type="arc" order="32" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_FixedIncomeSecuritiesRiskMember" xlink:label="loc_imstiiFixedIncomeSecuritiesRiskMember_40" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiFixedIncomeSecuritiesRiskMember_40" xlink:type="arc" order="42" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_CreditRiskMember" xlink:label="loc_imstiiCreditRiskMember_50" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiCreditRiskMember_50" xlink:type="arc" order="52" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PrepaymentRiskMember" xlink:label="loc_imstiiPrepaymentRiskMember_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPrepaymentRiskMember_60" xlink:type="arc" order="62" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_BankLoanRiskMember" xlink:label="loc_imstiiBankLoanRiskMember_70" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiBankLoanRiskMember_70" xlink:type="arc" order="72" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ForeignInvestmentRiskMember" xlink:label="loc_imstiiForeignInvestmentRiskMember_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiForeignInvestmentRiskMember_80" xlink:type="arc" order="82" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_EquityRiskMember" xlink:label="loc_imstiiEquityRiskMember_90" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiEquityRiskMember_90" xlink:type="arc" order="92" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ConvertibleSecuritiesRiskMember" xlink:label="loc_imstiiConvertibleSecuritiesRiskMember_100" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiConvertibleSecuritiesRiskMember_100" xlink:type="arc" order="102" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_WarrantsAndRightsRiskMember" xlink:label="loc_imstiiWarrantsAndRightsRiskMember_110" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiWarrantsAndRightsRiskMember_110" xlink:type="arc" order="112" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_SmallCapAndMidCapCompanyRiskMember" xlink:label="loc_imstiiSmallCapAndMidCapCompanyRiskMember_120" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiSmallCapAndMidCapCompanyRiskMember_120" xlink:type="arc" order="122" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LargeCapCompanyRiskMember" xlink:label="loc_imstiiLargeCapCompanyRiskMember_130" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLargeCapCompanyRiskMember_130" xlink:type="arc" order="132" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_SectorFocusRiskMember" xlink:label="loc_imstiiSectorFocusRiskMember_140" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiSectorFocusRiskMember_140" xlink:type="arc" order="142" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_InterestRateRiskMember" xlink:label="loc_imstiiInterestRateRiskMember_150" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiInterestRateRiskMember_150" xlink:type="arc" order="152" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_GovernmentSponsoredEntitiesRiskMember" xlink:label="loc_imstiiGovernmentSponsoredEntitiesRiskMember_160" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiGovernmentSponsoredEntitiesRiskMember_160" xlink:type="arc" order="162" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_MoneyMarketFundRiskMember" xlink:label="loc_imstiiMoneyMarketFundRiskMember_170" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiMoneyMarketFundRiskMember_170" xlink:type="arc" order="172" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PrivatePlacementsRiskMember" xlink:label="loc_imstiiPrivatePlacementsRiskMember_180" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPrivatePlacementsRiskMember_180" xlink:type="arc" order="182" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Rule144AAndOtherExemptedSecuritiesRiskMember" xlink:label="loc_imstiiRule144AAndOtherExemptedSecuritiesRiskMember_190" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiRule144AAndOtherExemptedSecuritiesRiskMember_190" xlink:type="arc" order="192" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LiquidityRiskMember" xlink:label="loc_imstiiLiquidityRiskMember_200" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLiquidityRiskMember_200" xlink:type="arc" order="202" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PortfolioTurnoverRiskMember" xlink:label="loc_imstiiPortfolioTurnoverRiskMember_210" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPortfolioTurnoverRiskMember_210" xlink:type="arc" order="212" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ManagementAndStrategyRiskMember" xlink:label="loc_imstiiManagementAndStrategyRiskMember_220" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiManagementAndStrategyRiskMember_220" xlink:type="arc" order="222" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LimitedOperatingHistoryMember" xlink:label="loc_imstiiLimitedOperatingHistoryMember_230" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLimitedOperatingHistoryMember_230" xlink:type="arc" order="232" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_RecentMarketEventsMember" xlink:label="loc_imstiiRecentMarketEventsMember_240" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiRecentMarketEventsMember_240" xlink:type="arc" order="242" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_CybersecurityRiskMember" xlink:label="loc_imstiiCybersecurityRiskMember_250" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiCybersecurityRiskMember_250" xlink:type="arc" order="252" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskReturnHeading" xlink:label="loc_rrRiskReturnHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskReturnHeading_270" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ObjectiveHeading" xlink:label="loc_rrObjectiveHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrObjectiveHeading_270" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ObjectivePrimaryTextBlock" xlink:label="loc_rrObjectivePrimaryTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrObjectivePrimaryTextBlock_270" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ObjectiveSecondaryTextBlock" xlink:label="loc_rrObjectiveSecondaryTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrObjectiveSecondaryTextBlock_270" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseHeading" xlink:label="loc_rrExpenseHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseHeading_270" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseNarrativeTextBlock" xlink:label="loc_rrExpenseNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseNarrativeTextBlock_270" xlink:type="arc" order="5" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareholderFeesCaption" xlink:label="loc_rrShareholderFeesCaption_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrShareholderFeesCaption_270" xlink:type="arc" order="6" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareholderFeesTableTextBlock" xlink:label="loc_rrShareholderFeesTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrShareholderFeesTableTextBlock_270" xlink:type="arc" order="7" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_OperatingExpensesCaption" xlink:label="loc_rrOperatingExpensesCaption_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrOperatingExpensesCaption_270" xlink:type="arc" order="8" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualFundOperatingExpensesTableTextBlock" xlink:label="loc_rrAnnualFundOperatingExpensesTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualFundOperatingExpensesTableTextBlock_270" xlink:type="arc" order="9" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseFootnotesTextBlock" xlink:label="loc_rrExpenseFootnotesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseFootnotesTextBlock_270" xlink:type="arc" order="10" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpensesDeferredChargesTextBlock" xlink:label="loc_rrExpensesDeferredChargesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpensesDeferredChargesTextBlock_270" xlink:type="arc" order="11" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpensesRangeOfExchangeFeesTextBlock" xlink:label="loc_rrExpensesRangeOfExchangeFeesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpensesRangeOfExchangeFeesTextBlock_270" xlink:type="arc" order="12" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleHeading" xlink:label="loc_rrExpenseExampleHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleHeading_270" xlink:type="arc" order="13" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleByYearHeading" xlink:label="loc_rrExpenseExampleByYearHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleByYearHeading_270" xlink:type="arc" order="14" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleNarrativeTextBlock" xlink:label="loc_rrExpenseExampleNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleNarrativeTextBlock_270" xlink:type="arc" order="15" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleByYearCaption" xlink:label="loc_rrExpenseExampleByYearCaption_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleByYearCaption_270" xlink:type="arc" order="16" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleWithRedemptionTableTextBlock" xlink:label="loc_rrExpenseExampleWithRedemptionTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleWithRedemptionTableTextBlock_270" xlink:type="arc" order="17" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleNoRedemptionNarrativeTextBlock" xlink:label="loc_rrExpenseExampleNoRedemptionNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleNoRedemptionNarrativeTextBlock_270" xlink:type="arc" order="18" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleNoRedemptionByYearCaption" xlink:label="loc_rrExpenseExampleNoRedemptionByYearCaption_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleNoRedemptionByYearCaption_270" xlink:type="arc" order="19" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleNoRedemptionTableTextBlock" xlink:label="loc_rrExpenseExampleNoRedemptionTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleNoRedemptionTableTextBlock_270" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleFootnotesTextBlock" xlink:label="loc_rrExpenseExampleFootnotesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleFootnotesTextBlock_270" xlink:type="arc" order="21" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleClosingTextBlock" xlink:label="loc_rrExpenseExampleClosingTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleClosingTextBlock_270" xlink:type="arc" order="22" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PortfolioTurnoverHeading" xlink:label="loc_rrPortfolioTurnoverHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPortfolioTurnoverHeading_270" xlink:type="arc" order="23" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PortfolioTurnoverTextBlock" xlink:label="loc_rrPortfolioTurnoverTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPortfolioTurnoverTextBlock_270" xlink:type="arc" order="24" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_StrategyHeading" xlink:label="loc_rrStrategyHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrStrategyHeading_270" xlink:type="arc" order="25" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_StrategyNarrativeTextBlock" xlink:label="loc_rrStrategyNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrStrategyNarrativeTextBlock_270" xlink:type="arc" order="26" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskHeading" xlink:label="loc_rrRiskHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskHeading_270" xlink:type="arc" order="27" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskNarrativeTextBlock" xlink:label="loc_rrRiskNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskNarrativeTextBlock_270" xlink:type="arc" order="28" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskTextBlock" xlink:label="loc_rrRiskTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskTextBlock_270" xlink:type="arc" order="29" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskFootnotesTextBlock" xlink:label="loc_rrRiskFootnotesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskFootnotesTextBlock_270" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RiskClosingTextBlock" xlink:label="loc_rrRiskClosingTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRiskClosingTextBlock_270" xlink:type="arc" order="31" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartAndPerformanceTableHeading" xlink:label="loc_rrBarChartAndPerformanceTableHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartAndPerformanceTableHeading_270" xlink:type="arc" order="32" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceNarrativeTextBlock" xlink:label="loc_rrPerformanceNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceNarrativeTextBlock_270" xlink:type="arc" order="33" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartNarrativeTextBlock" xlink:label="loc_rrBarChartNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartNarrativeTextBlock_270" xlink:type="arc" order="34" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartHeading" xlink:label="loc_rrBarChartHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartHeading_270" xlink:type="arc" order="35" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartTableTextBlock" xlink:label="loc_rrBarChartTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartTableTextBlock_270" xlink:type="arc" order="36" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartFootnotesTextBlock" xlink:label="loc_rrBarChartFootnotesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartFootnotesTextBlock_270" xlink:type="arc" order="37" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_BarChartClosingTextBlock" xlink:label="loc_rrBarChartClosingTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrBarChartClosingTextBlock_270" xlink:type="arc" order="38" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceTableHeading" xlink:label="loc_rrPerformanceTableHeading_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceTableHeading_270" xlink:type="arc" order="39" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceTableNarrativeTextBlock" xlink:label="loc_rrPerformanceTableNarrativeTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceTableNarrativeTextBlock_270" xlink:type="arc" order="40" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceTableTextBlock" xlink:label="loc_rrPerformanceTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceTableTextBlock_270" xlink:type="arc" order="41" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MarketIndexPerformanceTableTextBlock" xlink:label="loc_rrMarketIndexPerformanceTableTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMarketIndexPerformanceTableTextBlock_270" xlink:type="arc" order="42" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceTableFootnotesTextBlock" xlink:label="loc_rrPerformanceTableFootnotesTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceTableFootnotesTextBlock_270" xlink:type="arc" order="43" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceTableClosingTextBlock" xlink:label="loc_rrPerformanceTableClosingTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrPerformanceTableClosingTextBlock_270" xlink:type="arc" order="44" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_SupplementToProspectusTextBlock" xlink:label="loc_rrSupplementToProspectusTextBlock_270" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrSupplementToProspectusTextBlock_270" xlink:type="arc" order="45" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/ShareholderFeesData" xlink:title="00000004 - Schedule - Shareholder Fees">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusShareClassAxis" xlink:label="loc_rrProspectusShareClassAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrProspectusShareClassAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain" xlink:type="arc" order="60" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain_10" xlink:type="arc" order="61" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareholderFeesColumnName" xlink:label="loc_rrShareholderFeesColumnName_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrShareholderFeesColumnName_20" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_WireFee" xlink:label="loc_imstiiWireFee_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_imstiiWireFee_20" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_OvernightCheckDeliveryFee" xlink:label="loc_imstiiOvernightCheckDeliveryFee_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_imstiiOvernightCheckDeliveryFee_20" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumCumulativeSalesChargeOverOfferingPrice" xlink:label="loc_rrMaximumCumulativeSalesChargeOverOfferingPrice_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumCumulativeSalesChargeOverOfferingPrice_20" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumCumulativeSalesChargeOverOther" xlink:label="loc_rrMaximumCumulativeSalesChargeOverOther_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumCumulativeSalesChargeOverOther_20" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumSalesChargeImposedOnPurchasesOverOfferingPrice" xlink:label="loc_rrMaximumSalesChargeImposedOnPurchasesOverOfferingPrice_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumSalesChargeImposedOnPurchasesOverOfferingPrice_20" xlink:type="arc" order="5" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumDeferredSalesChargeOverOfferingPrice" xlink:label="loc_rrMaximumDeferredSalesChargeOverOfferingPrice_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumDeferredSalesChargeOverOfferingPrice_20" xlink:type="arc" order="6" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumDeferredSalesChargeOverOther" xlink:label="loc_rrMaximumDeferredSalesChargeOverOther_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumDeferredSalesChargeOverOther_20" xlink:type="arc" order="7" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumSalesChargeOnReinvestedDividendsAndDistributionsOverOther" xlink:label="loc_rrMaximumSalesChargeOnReinvestedDividendsAndDistributionsOverOther_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumSalesChargeOnReinvestedDividendsAndDistributionsOverOther_20" xlink:type="arc" order="8" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RedemptionFeeOverRedemption" xlink:label="loc_rrRedemptionFeeOverRedemption_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRedemptionFeeOverRedemption_20" xlink:type="arc" order="9" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_RedemptionFee" xlink:label="loc_rrRedemptionFee_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrRedemptionFee_20" xlink:type="arc" order="10" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExchangeFeeOverRedemption" xlink:label="loc_rrExchangeFeeOverRedemption_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExchangeFeeOverRedemption_20" xlink:type="arc" order="11" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExchangeFee" xlink:label="loc_rrExchangeFee_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExchangeFee_20" xlink:type="arc" order="12" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumAccountFeeOverAssets" xlink:label="loc_rrMaximumAccountFeeOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumAccountFeeOverAssets_20" xlink:type="arc" order="13" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_MaximumAccountFee" xlink:label="loc_rrMaximumAccountFee_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrMaximumAccountFee_20" xlink:type="arc" order="14" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareholderFeeOther" xlink:label="loc_rrShareholderFeeOther_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrShareholderFeeOther_20" xlink:type="arc" order="15" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/OperatingExpensesData" xlink:title="00000005 - Schedule - Annual Fund Operating Expenses">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusShareClassAxis" xlink:label="loc_rrProspectusShareClassAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrProspectusShareClassAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain" xlink:type="arc" order="60" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain_10" xlink:type="arc" order="61" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_OperatingExpensesColumnName" xlink:label="loc_rrOperatingExpensesColumnName_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrOperatingExpensesColumnName_20" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ManagementFeesOverAssets" xlink:label="loc_rrManagementFeesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrManagementFeesOverAssets_20" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_DistributionAndService12b1FeesOverAssets" xlink:label="loc_rrDistributionAndService12b1FeesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrDistributionAndService12b1FeesOverAssets_20" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_DistributionOrSimilarNon12b1FeesOverAssets" xlink:label="loc_rrDistributionOrSimilarNon12b1FeesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrDistributionOrSimilarNon12b1FeesOverAssets_20" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_Component1OtherExpensesOverAssets" xlink:label="loc_rrComponent1OtherExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrComponent1OtherExpensesOverAssets_20" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_Component2OtherExpensesOverAssets" xlink:label="loc_rrComponent2OtherExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrComponent2OtherExpensesOverAssets_20" xlink:type="arc" order="5" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_Component3OtherExpensesOverAssets" xlink:label="loc_rrComponent3OtherExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrComponent3OtherExpensesOverAssets_20" xlink:type="arc" order="6" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_OtherExpensesOverAssets" xlink:label="loc_rrOtherExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrOtherExpensesOverAssets_20" xlink:type="arc" order="7" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AcquiredFundFeesAndExpensesOverAssets" xlink:label="loc_rrAcquiredFundFeesAndExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAcquiredFundFeesAndExpensesOverAssets_20" xlink:type="arc" order="8" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpensesOverAssets" xlink:label="loc_rrExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpensesOverAssets_20" xlink:type="arc" order="9" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_FeeWaiverOrReimbursementOverAssets" xlink:label="loc_rrFeeWaiverOrReimbursementOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrFeeWaiverOrReimbursementOverAssets_20" xlink:type="arc" order="10" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_NetExpensesOverAssets" xlink:label="loc_rrNetExpensesOverAssets_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrNetExpensesOverAssets_20" xlink:type="arc" order="11" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/ExpenseExample" xlink:title="00000006 - Schedule - Expense Example {Transposed}">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusShareClassAxis" xlink:label="loc_rrProspectusShareClassAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrProspectusShareClassAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain" xlink:type="arc" order="60" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain_10" xlink:type="arc" order="61" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleByYearColumnName" xlink:label="loc_rrExpenseExampleByYearColumnName_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleByYearColumnName_20" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleYear01" xlink:label="loc_rrExpenseExampleYear01_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleYear01_20" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleYear03" xlink:label="loc_rrExpenseExampleYear03_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleYear03_20" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleYear05" xlink:label="loc_rrExpenseExampleYear05_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleYear05_20" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ExpenseExampleYear10" xlink:label="loc_rrExpenseExampleYear10_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrExpenseExampleYear10_20" xlink:type="arc" order="4" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/ExpenseExampleNoRedemption" xlink:title="00000007 - Schedule - Expense Example, No Redemption {Transposed}" />
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/BarChartData" xlink:title="00000008 - Schedule - Annual Total Returns">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusShareClassAxis" xlink:label="loc_rrProspectusShareClassAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrProspectusShareClassAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain" xlink:type="arc" order="60" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain_10" xlink:type="arc" order="61" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturnCaption" xlink:label="loc_rrAnnualReturnCaption_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturnCaption_20" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturnColumnName" xlink:label="loc_rrAnnualReturnColumnName_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturnColumnName_20" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturnInceptionDate" xlink:label="loc_rrAnnualReturnInceptionDate_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturnInceptionDate_20" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1990" xlink:label="loc_rrAnnualReturn1990_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1990_20" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1991" xlink:label="loc_rrAnnualReturn1991_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1991_20" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1992" xlink:label="loc_rrAnnualReturn1992_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1992_20" xlink:type="arc" order="5" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1993" xlink:label="loc_rrAnnualReturn1993_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1993_20" xlink:type="arc" order="6" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1994" xlink:label="loc_rrAnnualReturn1994_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1994_20" xlink:type="arc" order="7" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1995" xlink:label="loc_rrAnnualReturn1995_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1995_20" xlink:type="arc" order="8" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1996" xlink:label="loc_rrAnnualReturn1996_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1996_20" xlink:type="arc" order="9" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1997" xlink:label="loc_rrAnnualReturn1997_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1997_20" xlink:type="arc" order="10" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1998" xlink:label="loc_rrAnnualReturn1998_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1998_20" xlink:type="arc" order="11" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn1999" xlink:label="loc_rrAnnualReturn1999_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn1999_20" xlink:type="arc" order="12" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2000" xlink:label="loc_rrAnnualReturn2000_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2000_20" xlink:type="arc" order="13" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2001" xlink:label="loc_rrAnnualReturn2001_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2001_20" xlink:type="arc" order="14" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2002" xlink:label="loc_rrAnnualReturn2002_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2002_20" xlink:type="arc" order="15" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2003" xlink:label="loc_rrAnnualReturn2003_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2003_20" xlink:type="arc" order="16" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2004" xlink:label="loc_rrAnnualReturn2004_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2004_20" xlink:type="arc" order="17" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2005" xlink:label="loc_rrAnnualReturn2005_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2005_20" xlink:type="arc" order="18" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2006" xlink:label="loc_rrAnnualReturn2006_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2006_20" xlink:type="arc" order="19" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2007" xlink:label="loc_rrAnnualReturn2007_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2007_20" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2008" xlink:label="loc_rrAnnualReturn2008_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2008_20" xlink:type="arc" order="21" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2009" xlink:label="loc_rrAnnualReturn2009_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2009_20" xlink:type="arc" order="22" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2010" xlink:label="loc_rrAnnualReturn2010_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2010_20" xlink:type="arc" order="23" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2011" xlink:label="loc_rrAnnualReturn2011_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2011_20" xlink:type="arc" order="24" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2012" xlink:label="loc_rrAnnualReturn2012_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2012_20" xlink:type="arc" order="25" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2013" xlink:label="loc_rrAnnualReturn2013_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2013_20" xlink:type="arc" order="26" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2014" xlink:label="loc_rrAnnualReturn2014_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2014_20" xlink:type="arc" order="27" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2015" xlink:label="loc_rrAnnualReturn2015_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2015_20" xlink:type="arc" order="28" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2016" xlink:label="loc_rrAnnualReturn2016_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2016_20" xlink:type="arc" order="29" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2017" xlink:label="loc_rrAnnualReturn2017_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2017_20" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2018" xlink:label="loc_rrAnnualReturn2018_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2018_20" xlink:type="arc" order="31" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2019" xlink:label="loc_rrAnnualReturn2019_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2019_20" xlink:type="arc" order="32" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2020" xlink:label="loc_rrAnnualReturn2020_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2020_20" xlink:type="arc" order="33" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2021" xlink:label="loc_rrAnnualReturn2021_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2021_20" xlink:type="arc" order="34" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2022" xlink:label="loc_rrAnnualReturn2022_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2022_20" xlink:type="arc" order="35" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2023" xlink:label="loc_rrAnnualReturn2023_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2023_20" xlink:type="arc" order="36" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2024" xlink:label="loc_rrAnnualReturn2024_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2024_20" xlink:type="arc" order="37" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AnnualReturn2025" xlink:label="loc_rrAnnualReturn2025_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAnnualReturn2025_20" xlink:type="arc" order="38" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/PerformanceTableData" xlink:title="00000009 - Schedule - Average Annual Total Returns {Transposed}">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusLineItems" xlink:label="loc_rrProspectusLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusTable" xlink:label="loc_rrProspectusTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrProspectusTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_LegalEntityAxis" xlink:label="loc_deiLegalEntityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_deiLegalEntityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_deiLegalEntityAxis" xlink:to="loc_deiEntityDomain_10" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ProspectusShareClassAxis" xlink:label="loc_rrProspectusShareClassAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrProspectusShareClassAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain" xlink:type="arc" order="70" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrProspectusShareClassAxis" xlink:to="loc_rrShareClassDomain_10" xlink:type="arc" order="71" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceMeasureAxis" xlink:label="loc_rrPerformanceMeasureAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_rrProspectusTable" xlink:to="loc_rrPerformanceMeasureAxis" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceMeasureDomain" xlink:label="loc_rrPerformanceMeasureDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_rrPerformanceMeasureAxis" xlink:to="loc_rrPerformanceMeasureDomain" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceMeasureDomain" xlink:label="loc_rrPerformanceMeasureDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_rrPerformanceMeasureAxis" xlink:to="loc_rrPerformanceMeasureDomain_10" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AfterTaxesOnDistributionsMember" xlink:label="loc_rrAfterTaxesOnDistributionsMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_rrAfterTaxesOnDistributionsMember_10" xlink:type="arc" order="13" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AfterTaxesOnDistributionsAndSalesMember" xlink:label="loc_rrAfterTaxesOnDistributionsAndSalesMember_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_rrAfterTaxesOnDistributionsAndSalesMember_20" xlink:type="arc" order="23" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Index1Member" xlink:label="loc_imstiiIndex1Member_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_imstiiIndex1Member_30" xlink:type="arc" order="32" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Index2Member" xlink:label="loc_imstiiIndex2Member_40" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_imstiiIndex2Member_40" xlink:type="arc" order="42" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnLabel" xlink:label="loc_rrAverageAnnualReturnLabel_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnLabel_60" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnYear01" xlink:label="loc_rrAverageAnnualReturnYear01_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnYear01_60" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnYear05" xlink:label="loc_rrAverageAnnualReturnYear05_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnYear05_60" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnYear10" xlink:label="loc_rrAverageAnnualReturnYear10_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnYear10_60" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnSinceInception" xlink:label="loc_rrAverageAnnualReturnSinceInception_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnSinceInception_60" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AverageAnnualReturnInceptionDate" xlink:label="loc_rrAverageAnnualReturnInceptionDate_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrProspectusLineItems" xlink:to="loc_rrAverageAnnualReturnInceptionDate_60" xlink:type="arc" order="5" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://imstii/role/RiskReturnDetail" xlink:title="00000010 - Document - Risk/Return Detail {Unlabeled}" />
    <link:definitionLink xlink:type="extended" xlink:role="http://xbrl.sec.gov/rr/role/RiskReturnDetailData" xlink:title="00000011 - Disclosure - Risk/Return Detail Data {Elements}" />
    <link:definitionLink xlink:type="extended" xlink:role="http://xbrl.sec.gov/rr/role/Series">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2023/dei-2023.xsd#dei_EntityDomain" xlink:label="loc_deiEntityDomain" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_S000077098Member" xlink:label="loc_imstiiS000077098Member" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_deiEntityDomain" xlink:to="loc_imstiiS000077098Member" xlink:type="arc" order="0" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://xbrl.sec.gov/rr/role/Class">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_ShareClassDomain" xlink:label="loc_rrShareClassDomain" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_C000237262Member" xlink:label="loc_imstiiC000237262Member" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrShareClassDomain" xlink:to="loc_imstiiC000237262Member" xlink:type="arc" order="500" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://xbrl.sec.gov/rr/role/PerformanceMeasure">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_PerformanceMeasureDomain" xlink:label="loc_rrPerformanceMeasureDomain" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Index1Member" xlink:label="loc_imstiiIndex1Member" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_imstiiIndex1Member" xlink:type="arc" order="2000" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Index2Member" xlink:label="loc_imstiiIndex2Member" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrPerformanceMeasureDomain" xlink:to="loc_imstiiIndex2Member" xlink:type="arc" order="2010" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://xbrl.sec.gov/rr/role/Risk">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/rr/2023/rr-2023.xsd#rr_AllRisksMember" xlink:label="loc_rrAllRisksMember" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_MarketRiskMember" xlink:label="loc_imstiiMarketRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiMarketRiskMember" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_HighYieldJunkBondRiskMember" xlink:label="loc_imstiiHighYieldJunkBondRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiHighYieldJunkBondRiskMember" xlink:type="arc" order="10" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_FixedIncomeSecuritiesRiskMember" xlink:label="loc_imstiiFixedIncomeSecuritiesRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiFixedIncomeSecuritiesRiskMember" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_CreditRiskMember" xlink:label="loc_imstiiCreditRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiCreditRiskMember" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PrepaymentRiskMember" xlink:label="loc_imstiiPrepaymentRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPrepaymentRiskMember" xlink:type="arc" order="40" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_BankLoanRiskMember" xlink:label="loc_imstiiBankLoanRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiBankLoanRiskMember" xlink:type="arc" order="50" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ForeignInvestmentRiskMember" xlink:label="loc_imstiiForeignInvestmentRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiForeignInvestmentRiskMember" xlink:type="arc" order="60" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_EquityRiskMember" xlink:label="loc_imstiiEquityRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiEquityRiskMember" xlink:type="arc" order="70" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ConvertibleSecuritiesRiskMember" xlink:label="loc_imstiiConvertibleSecuritiesRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiConvertibleSecuritiesRiskMember" xlink:type="arc" order="80" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_WarrantsAndRightsRiskMember" xlink:label="loc_imstiiWarrantsAndRightsRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiWarrantsAndRightsRiskMember" xlink:type="arc" order="90" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_SmallCapAndMidCapCompanyRiskMember" xlink:label="loc_imstiiSmallCapAndMidCapCompanyRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiSmallCapAndMidCapCompanyRiskMember" xlink:type="arc" order="100" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LargeCapCompanyRiskMember" xlink:label="loc_imstiiLargeCapCompanyRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLargeCapCompanyRiskMember" xlink:type="arc" order="110" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_SectorFocusRiskMember" xlink:label="loc_imstiiSectorFocusRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiSectorFocusRiskMember" xlink:type="arc" order="120" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_InterestRateRiskMember" xlink:label="loc_imstiiInterestRateRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiInterestRateRiskMember" xlink:type="arc" order="130" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_GovernmentSponsoredEntitiesRiskMember" xlink:label="loc_imstiiGovernmentSponsoredEntitiesRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiGovernmentSponsoredEntitiesRiskMember" xlink:type="arc" order="140" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_MoneyMarketFundRiskMember" xlink:label="loc_imstiiMoneyMarketFundRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiMoneyMarketFundRiskMember" xlink:type="arc" order="150" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PrivatePlacementsRiskMember" xlink:label="loc_imstiiPrivatePlacementsRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPrivatePlacementsRiskMember" xlink:type="arc" order="160" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_Rule144AAndOtherExemptedSecuritiesRiskMember" xlink:label="loc_imstiiRule144AAndOtherExemptedSecuritiesRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiRule144AAndOtherExemptedSecuritiesRiskMember" xlink:type="arc" order="170" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LiquidityRiskMember" xlink:label="loc_imstiiLiquidityRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLiquidityRiskMember" xlink:type="arc" order="180" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_PortfolioTurnoverRiskMember" xlink:label="loc_imstiiPortfolioTurnoverRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiPortfolioTurnoverRiskMember" xlink:type="arc" order="190" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_ManagementAndStrategyRiskMember" xlink:label="loc_imstiiManagementAndStrategyRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiManagementAndStrategyRiskMember" xlink:type="arc" order="200" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_LimitedOperatingHistoryMember" xlink:label="loc_imstiiLimitedOperatingHistoryMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiLimitedOperatingHistoryMember" xlink:type="arc" order="210" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_RecentMarketEventsMember" xlink:label="loc_imstiiRecentMarketEventsMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiRecentMarketEventsMember" xlink:type="arc" order="220" />
      <link:loc xlink:type="locator" xlink:href="imstii-20250228.xsd#imstii_CybersecurityRiskMember" xlink:label="loc_imstiiCybersecurityRiskMember" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_rrAllRisksMember" xlink:to="loc_imstiiCybersecurityRiskMember" xlink:type="arc" order="230" />
    </link:definitionLink>
</link:linkbase>
