<?xml version="1.0" encoding="US-ASCII" standalone="no"?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 6.1a -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: https://www.novaworks.com -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrli="http://www.xbrl.org/2003/instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#Cover" roleURI="http://n2off.com/role/Cover" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InterimBalanceSheets" roleURI="http://n2off.com/role/InterimBalanceSheets" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InterimBalanceSheetsParenthetical" roleURI="http://n2off.com/role/InterimBalanceSheetsParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InterimStatementsOfComprehensiveLoss" roleURI="http://n2off.com/role/InterimStatementsOfComprehensiveLoss" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InterimStatementsOfChangesInStockholdersEquity" roleURI="http://n2off.com/role/InterimStatementsOfChangesInStockholdersEquity" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InterimStatementsOfCashFlows" roleURI="http://n2off.com/role/InterimStatementsOfCashFlows" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#General" roleURI="http://n2off.com/role/General" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SummaryOfSignificantAccountingPoliciesAndBasisOfPresentation" roleURI="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentation" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#OtherCurrentAssets" roleURI="http://n2off.com/role/OtherCurrentAssets" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#MitocarexTransaction" roleURI="http://n2off.com/role/MitocarexTransaction" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#PropertyAndEquipmentNet" roleURI="http://n2off.com/role/PropertyAndEquipmentNet" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InvestmentInNonconsolidatedAffiliate" roleURI="http://n2off.com/role/InvestmentInNonconsolidatedAffiliate" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InvestmentAndLoanToSolterra" roleURI="http://n2off.com/role/InvestmentAndLoanToSolterra" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SolarPhotovolaicJointVentureProject" roleURI="http://n2off.com/role/SolarPhotovolaicJointVentureProject" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ConsolidatedEntity" roleURI="http://n2off.com/role/ConsolidatedEntity" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#OtherLiabilities" roleURI="http://n2off.com/role/OtherLiabilities" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CommitmentAndContingentLiabilities" roleURI="http://n2off.com/role/CommitmentAndContingentLiabilities" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#Leases" roleURI="http://n2off.com/role/Leases" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CommonStockAndWarrants" roleURI="http://n2off.com/role/CommonStockAndWarrants" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#StockOptions" roleURI="http://n2off.com/role/StockOptions" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CostOfSales" roleURI="http://n2off.com/role/CostOfSales" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ResearchAndDevelopmentExpenses" roleURI="http://n2off.com/role/ResearchAndDevelopmentExpenses" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SellingAndMarketingExpenses" roleURI="http://n2off.com/role/SellingAndMarketingExpenses" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#GeneralAndAdministrativeExpenses" roleURI="http://n2off.com/role/GeneralAndAdministrativeExpenses" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#FinancingExpensesIncomeNet" roleURI="http://n2off.com/role/FinancingExpensesIncomeNet" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#IncomeTax" roleURI="http://n2off.com/role/IncomeTax" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#LossPerShare" roleURI="http://n2off.com/role/LossPerShare" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#RelatedParties" roleURI="http://n2off.com/role/RelatedParties" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SegmentReporting" roleURI="http://n2off.com/role/SegmentReporting" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SubsequentEvents" roleURI="http://n2off.com/role/SubsequentEvents" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CreditFacility" roleURI="http://n2off.com/role/CreditFacility" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#DiscontinuedOperations" roleURI="http://n2off.com/role/DiscontinuedOperations" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationPolicies" roleURI="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationPolicies" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationTables" roleURI="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#OtherCurrentAssetsTables" roleURI="http://n2off.com/role/OtherCurrentAssetsTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#PropertyAndEquipmentNetTables" roleURI="http://n2off.com/role/PropertyAndEquipmentNetTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InvestmentInNonconsolidatedAffiliateTables" roleURI="http://n2off.com/role/InvestmentInNonconsolidatedAffiliateTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#OtherLiabilitiesTables" roleURI="http://n2off.com/role/OtherLiabilitiesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CommitmentAndContingentLiabilitiesTables" roleURI="http://n2off.com/role/CommitmentAndContingentLiabilitiesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#LeasesTables" roleURI="http://n2off.com/role/LeasesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#StockOptionsTables" roleURI="http://n2off.com/role/StockOptionsTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CostOfSalesTables" roleURI="http://n2off.com/role/CostOfSalesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ResearchAndDevelopmentExpensesTables" roleURI="http://n2off.com/role/ResearchAndDevelopmentExpensesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SellingAndMarketingExpensesTables" roleURI="http://n2off.com/role/SellingAndMarketingExpensesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#GeneralAndAdministrativeExpensesTables" roleURI="http://n2off.com/role/GeneralAndAdministrativeExpensesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#FinancingExpensesIncomeNetTables" roleURI="http://n2off.com/role/FinancingExpensesIncomeNetTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#IncomeTaxTables" roleURI="http://n2off.com/role/IncomeTaxTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#LossPerShareTables" roleURI="http://n2off.com/role/LossPerShareTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#RelatedPartiesTables" roleURI="http://n2off.com/role/RelatedPartiesTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SegmentReportingTables" roleURI="http://n2off.com/role/SegmentReportingTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CreditFacilityTables" roleURI="http://n2off.com/role/CreditFacilityTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#DiscontinuedOperationsTables" roleURI="http://n2off.com/role/DiscontinuedOperationsTables" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#GeneralDetailsNarrative" roleURI="http://n2off.com/role/GeneralDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfPropertyPlantAndEquipmentDepreciationRatesDetails" roleURI="http://n2off.com/role/ScheduleOfPropertyPlantAndEquipmentDepreciationRatesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfFairValueAssetsOnRecurringBasisDetails" roleURI="http://n2off.com/role/ScheduleOfFairValueAssetsOnRecurringBasisDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfChangesInFairValueOfAssetsDetails" roleURI="http://n2off.com/role/ScheduleOfChangesInFairValueOfAssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationDetailsNarrative" roleURI="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfOtherCurrentAssetsDetails" roleURI="http://n2off.com/role/ScheduleOfOtherCurrentAssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#MitocarexTransactionDetailsNarrative" roleURI="http://n2off.com/role/MitocarexTransactionDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfPropertyPlantAndEquipmentDetails" roleURI="http://n2off.com/role/ScheduleOfPropertyPlantAndEquipmentDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#PropertyAndEquipmentNetDetailsNarrative" roleURI="http://n2off.com/role/PropertyAndEquipmentNetDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfFairValueConversionDetails" roleURI="http://n2off.com/role/ScheduleOfFairValueConversionDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfEquityInvestmentDetails" roleURI="http://n2off.com/role/ScheduleOfEquityInvestmentDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InvestmentInNonconsolidatedAffiliateDetailsNarrative" roleURI="http://n2off.com/role/InvestmentInNonconsolidatedAffiliateDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#InvestmentAndLoanToSolterraDetailsNarrative" roleURI="http://n2off.com/role/InvestmentAndLoanToSolterraDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SolarPhotovolaicJointVentureProjectDetailsNarrative" roleURI="http://n2off.com/role/SolarPhotovolaicJointVentureProjectDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ConsolidatedEntityDetailsNarrative" roleURI="http://n2off.com/role/ConsolidatedEntityDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfOtherLiabilitiesDetails" roleURI="http://n2off.com/role/ScheduleOfOtherLiabilitiesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfWarrantAssumptionsUsedDetails" roleURI="http://n2off.com/role/ScheduleOfWarrantAssumptionsUsedDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CommitmentAndContingentLiabilitiesDetailsNarrative" roleURI="http://n2off.com/role/CommitmentAndContingentLiabilitiesDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfOperatingLeaseCostDetails" roleURI="http://n2off.com/role/ScheduleOfOperatingLeaseCostDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfSupplementalCashFlowInformationRelatedToOperatingLeaseDetails" roleURI="http://n2off.com/role/ScheduleOfSupplementalCashFlowInformationRelatedToOperatingLeaseDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfSupplementalBalanceSheetInformationRelatedToOperatingLeasesDetails" roleURI="http://n2off.com/role/ScheduleOfSupplementalBalanceSheetInformationRelatedToOperatingLeasesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfMinimumLeasePaymentsUnderNon-CancelableLeasesDetails" roleURI="http://n2off.com/role/ScheduleOfMinimumLeasePaymentsUnderNon-CancelableLeasesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#LeasesDetailsNarrative" roleURI="http://n2off.com/role/LeasesDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetailsParenthetical" roleURI="http://n2off.com/role/ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetailsParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CommonStockAndWarrantsDetailsNarrative" roleURI="http://n2off.com/role/CommonStockAndWarrantsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfEstimatedFairValueOfOptionsGrantedDetails" roleURI="http://n2off.com/role/ScheduleOfEstimatedFairValueOfOptionsGrantedDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfEstimatedFairValueOfOptionsGrantedDetailsParenthetical" roleURI="http://n2off.com/role/ScheduleOfEstimatedFairValueOfOptionsGrantedDetailsParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfStockOptionActivityDetails" roleURI="http://n2off.com/role/ScheduleOfStockOptionActivityDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#StockOptionsDetailsNarrative" roleURI="http://n2off.com/role/StockOptionsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfCostOfSalesDetails" roleURI="http://n2off.com/role/ScheduleOfCostOfSalesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfResearchAndDevelopmentExpensesDetails" roleURI="http://n2off.com/role/ScheduleOfResearchAndDevelopmentExpensesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfSellingAndMarketingExpensesDetails" roleURI="http://n2off.com/role/ScheduleOfSellingAndMarketingExpensesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfGeneralAndAdministrativeExpensesDetails" roleURI="http://n2off.com/role/ScheduleOfGeneralAndAdministrativeExpensesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfFinancingExpensesNetDetails" roleURI="http://n2off.com/role/ScheduleOfFinancingExpensesNetDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfIncomeTaxExpensesDetails" roleURI="http://n2off.com/role/ScheduleOfIncomeTaxExpensesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfDeferredTaxAssetsAndLiabilitiesDetails" roleURI="http://n2off.com/role/ScheduleOfDeferredTaxAssetsAndLiabilitiesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#IncomeTaxDetailsNarrative" roleURI="http://n2off.com/role/IncomeTaxDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfBasicAndDilutedLossPerShareDetails" roleURI="http://n2off.com/role/ScheduleOfBasicAndDilutedLossPerShareDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetails" roleURI="http://n2off.com/role/ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#RelatedPartiesDetailsNarrative" roleURI="http://n2off.com/role/RelatedPartiesDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfInformationRelatedToOperationsOfReportableOperatingSegmentsDetails" roleURI="http://n2off.com/role/ScheduleOfInformationRelatedToOperationsOfReportableOperatingSegmentsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfInformationOnSalesByGeographicDistributionDetails" roleURI="http://n2off.com/role/ScheduleOfInformationOnSalesByGeographicDistributionDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfSalesToCustomersDetails" roleURI="http://n2off.com/role/ScheduleOfSalesToCustomersDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfInformationOnLongLivedAssetsDetails" roleURI="http://n2off.com/role/ScheduleOfInformationOnLongLivedAssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SegmentReportingDetailsNarrative" roleURI="http://n2off.com/role/SegmentReportingDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfFairValueOfLevels3AssetsDetails" roleURI="http://n2off.com/role/ScheduleOfFairValueOfLevels3AssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#SubsequentEventsDetailsNarrative" roleURI="http://n2off.com/role/SubsequentEventsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfEstimatedFairValueOfWarrantSharesDetails" roleURI="http://n2off.com/role/ScheduleOfEstimatedFairValueOfWarrantSharesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#CreditFacilityDetailsNarrative" roleURI="http://n2off.com/role/CreditFacilityDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#ScheduleOfEstimatedFairValueOfWarrantSharesAssumptionsDetails" roleURI="http://n2off.com/role/ScheduleOfEstimatedFairValueOfWarrantSharesAssumptionsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="nito-20250630.xsd#DiscontinuedOperationsDetailsNarrative" roleURI="http://n2off.com/role/DiscontinuedOperationsDetailsNarrative" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/Cover" xlink:title="00000001 - Document - Cover" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InterimBalanceSheets" xlink:title="00000002 - Statement - Condensed Consolidated Interim Balance Sheets">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AssetsCurrent" xlink:label="loc_us-gaapAssetsCurrent" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_CashEquivalentAtCarryingValue" xlink:label="loc_NITOCashEquivalentAtCarryingValue" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_NITOCashEquivalentAtCarryingValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RestrictedCashCurrent" xlink:label="loc_us-gaapRestrictedCashCurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapRestrictedCashCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_MarketableSecuritiesCurrent" xlink:label="loc_us-gaapMarketableSecuritiesCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapMarketableSecuritiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccountsReceivableNetCurrent" xlink:label="loc_us-gaapAccountsReceivableNetCurrent" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapAccountsReceivableNetCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ShortTermLoanCurrent" xlink:label="loc_NITOShortTermLoanCurrent" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_NITOShortTermLoanCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InventoryNet" xlink:label="loc_us-gaapInventoryNet" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapInventoryNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PrepaidExpenseCurrent" xlink:label="loc_us-gaapPrepaidExpenseCurrent" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapPrepaidExpenseCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherAssetsCurrent" xlink:label="loc_us-gaapOtherAssetsCurrent" />
      <link:calculationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapOtherAssetsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:label="loc_us-gaapAssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent" />
      <link:calculationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapAssetsOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_Assets" xlink:label="loc_us-gaapAssets" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapAssetsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PrepaidExpenseNoncurrent" xlink:label="loc_us-gaapPrepaidExpenseNoncurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapPrepaidExpenseNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseRightOfUseAsset" xlink:label="loc_us-gaapOperatingLeaseRightOfUseAsset" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapOperatingLeaseRightOfUseAsset" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaapPropertyPlantAndEquipmentNet" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapPropertyPlantAndEquipmentNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EquityMethodInvestments" xlink:label="loc_us-gaapEquityMethodInvestments" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapEquityMethodInvestments" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_LongTermLoansNoncurrent" xlink:label="loc_NITOLongTermLoansNoncurrent" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_NITOLongTermLoansNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherAssetsNoncurrent" xlink:label="loc_us-gaapOtherAssetsNoncurrent" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapOtherAssetsNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_SolarProjectsUnderDevelopment" xlink:label="loc_NITOSolarProjectsUnderDevelopment" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_NITOSolarProjectsUnderDevelopment" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaapLiabilitiesCurrent" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccountsPayableCurrent" xlink:label="loc_us-gaapAccountsPayableCurrent" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapAccountsPayableCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_OtherLiability" xlink:label="loc_NITOOtherLiability" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_NITOOtherLiability" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherLiabilitiesCurrent" xlink:label="loc_us-gaapOtherLiabilitiesCurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapOtherLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_WarrantLiabilitiesCurrent" xlink:label="loc_NITOWarrantLiabilitiesCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_NITOWarrantLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:label="loc_us-gaapLiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapLiabilitiesOfDisposalGroupIncludingDiscontinuedOperationCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_Liabilities" xlink:label="loc_us-gaapLiabilities" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent" xlink:label="loc_us-gaapOperatingLeaseLiabilityNoncurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapOperatingLeaseLiabilityNoncurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LineOfCredit" xlink:label="loc_us-gaapLineOfCredit" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapLineOfCredit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_WarrantLiabilitiesNonCurrent" xlink:label="loc_NITOWarrantLiabilitiesNonCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_NITOWarrantLiabilitiesNonCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaapStockholdersEquity" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CommonStockValue" xlink:label="loc_us-gaapCommonStockValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapCommonStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PreferredStockValue" xlink:label="loc_us-gaapPreferredStockValue" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapPreferredStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="loc_us-gaapAdditionalPaidInCapital" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapAdditionalPaidInCapital" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossForeignCurrencyTranslationAdjustmentNetOfTax" xlink:label="loc_us-gaapAccumulatedOtherComprehensiveIncomeLossForeignCurrencyTranslationAdjustmentNetOfTax" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapAccumulatedOtherComprehensiveIncomeLossForeignCurrencyTranslationAdjustmentNetOfTax" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="loc_us-gaapRetainedEarningsAccumulatedDeficit" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapRetainedEarningsAccumulatedDeficit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest" xlink:label="loc_us-gaapStockholdersEquityIncludingPortionAttributableToNoncontrollingInterest" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquityIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapStockholdersEquity" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_MinorityInterest" xlink:label="loc_us-gaapMinorityInterest" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquityIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapMinorityInterest" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaapLiabilitiesAndStockholdersEquity" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapLiabilities" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapStockholdersEquityIncludingPortionAttributableToNoncontrollingInterest" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InterimBalanceSheetsParenthetical" xlink:title="00000003 - Statement - Condensed Consolidated Interim Balance Sheets (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InterimStatementsOfComprehensiveLoss" xlink:title="00000004 - Statement - Condensed Consolidated Interim Statements of Comprehensive Loss">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GrossProfit" xlink:label="loc_us-gaapGrossProfit" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax" xlink:label="loc_us-gaapRevenueFromContractWithCustomerExcludingAssessedTax" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapGrossProfit" xlink:to="loc_us-gaapRevenueFromContractWithCustomerExcludingAssessedTax" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CostOfGoodsAndServicesSold" xlink:label="loc_us-gaapCostOfGoodsAndServicesSold" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapGrossProfit" xlink:to="loc_us-gaapCostOfGoodsAndServicesSold" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingIncomeLoss" xlink:label="loc_us-gaapOperatingIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapGrossProfit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ResearchAndDevelopmentExpense" xlink:label="loc_us-gaapResearchAndDevelopmentExpense" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapResearchAndDevelopmentExpense" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_SellingAndMarketingExpense" xlink:label="loc_us-gaapSellingAndMarketingExpense" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapSellingAndMarketingExpense" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GeneralAndAdministrativeExpense" xlink:label="loc_us-gaapGeneralAndAdministrativeExpense" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingIncomeLoss" xlink:to="loc_us-gaapGeneralAndAdministrativeExpense" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:label="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapOperatingIncomeLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_FinancingIncomeExpensesNet" xlink:label="loc_NITOFinancingIncomeExpensesNet" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_NITOFinancingIncomeExpensesNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherNonoperatingIncomeExpense" xlink:label="loc_us-gaapOtherNonoperatingIncomeExpense" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapOtherNonoperatingIncomeExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GainLossOnInvestments" xlink:label="loc_us-gaapGainLossOnInvestments" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapGainLossOnInvestments" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProfitLoss" xlink:label="loc_us-gaapProfitLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapProfitLoss" xlink:to="loc_us-gaapIncomeLossFromContinuingOperationsIncludingPortionAttributableToNoncontrollingInterest" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeLossFromDiscontinuedOperationsNetOfTax" xlink:label="loc_us-gaapIncomeLossFromDiscontinuedOperationsNetOfTax" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapProfitLoss" xlink:to="loc_us-gaapIncomeLossFromDiscontinuedOperationsNetOfTax" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaapNetIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapProfitLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetIncomeLossAttributableToNoncontrollingInterest" xlink:label="loc_us-gaapNetIncomeLossAttributableToNoncontrollingInterest" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapNetIncomeLossAttributableToNoncontrollingInterest" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ComprehensiveIncomeLossNetOfTax" xlink:label="loc_NITOComprehensiveIncomeLossNetOfTax" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_NITOComprehensiveIncomeLossNetOfTax" xlink:to="loc_us-gaapProfitLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentBeforeTax" xlink:label="loc_us-gaapOtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentBeforeTax" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_NITOComprehensiveIncomeLossNetOfTax" xlink:to="loc_us-gaapOtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentBeforeTax" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ComprehensiveIncomeNetOfTaxAttributableToNoncontrollingInterest" xlink:label="loc_us-gaapComprehensiveIncomeNetOfTaxAttributableToNoncontrollingInterest" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapComprehensiveIncomeNetOfTaxAttributableToNoncontrollingInterest" xlink:to="loc_NITOComprehensiveIncomeLossNetOfTax" xlink:type="arc" weight="-1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapComprehensiveIncomeNetOfTaxAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapNetIncomeLossAttributableToNoncontrollingInterest" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToNoncontrollingInterest" xlink:label="loc_us-gaapOtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToNoncontrollingInterest" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapComprehensiveIncomeNetOfTaxAttributableToNoncontrollingInterest" xlink:to="loc_us-gaapOtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToNoncontrollingInterest" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InterimStatementsOfChangesInStockholdersEquity" xlink:title="00000005 - Statement - Condensed Consolidated Interim Statements of Changes in Stockholders&apos; Equity" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InterimStatementsOfCashFlows" xlink:title="00000006 - Statement - Condensed Consolidated Interim Statements of Cash Flows">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeLossFromContinuingOperations" xlink:label="loc_us-gaapIncomeLossFromContinuingOperations" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncomeLossFromContinuingOperations" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ProfitLossForThePeriod" xlink:label="loc_NITOProfitLossForThePeriod" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOProfitLossForThePeriod" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DepreciationAndAmortization" xlink:label="loc_us-gaapDepreciationAndAmortization" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapDepreciationAndAmortization" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IssuanceOfStockAndWarrantsForServicesOrClaims" xlink:label="loc_us-gaapIssuanceOfStockAndWarrantsForServicesOrClaims" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIssuanceOfStockAndWarrantsForServicesOrClaims" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NoncashContributionExpense" xlink:label="loc_us-gaapNoncashContributionExpense" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapNoncashContributionExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ShareBasedCompensation" xlink:label="loc_us-gaapShareBasedCompensation" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapShareBasedCompensation" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GainLossOnSaleOfPropertyPlantEquipment" xlink:label="loc_us-gaapGainLossOnSaleOfPropertyPlantEquipment" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapGainLossOnSaleOfPropertyPlantEquipment" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_GainFromStandbyEquityPurchaseAgreementI" xlink:label="loc_NITOGainFromStandbyEquityPurchaseAgreementI" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOGainFromStandbyEquityPurchaseAgreementI" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ExpensesFromStandbyEquityPurchaseAgreementIIAndPromissoryNote" xlink:label="loc_NITOExpensesFromStandbyEquityPurchaseAgreementIIAndPromissoryNote" />
      <link:calculationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOExpensesFromStandbyEquityPurchaseAgreementIIAndPromissoryNote" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_InterestOnLoan" xlink:label="loc_NITOInterestOnLoan" />
      <link:calculationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOInterestOnLoan" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ChangeInFairValueOfLoans" xlink:label="loc_NITOChangeInFairValueOfLoans" />
      <link:calculationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOChangeInFairValueOfLoans" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueAdjustmentOfWarrants" xlink:label="loc_us-gaapFairValueAdjustmentOfWarrants" />
      <link:calculationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapFairValueAdjustmentOfWarrants" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ChangeInFairValueOfCreditFacilityLiability" xlink:label="loc_NITOChangeInFairValueOfCreditFacilityLiability" />
      <link:calculationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOChangeInFairValueOfCreditFacilityLiability" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ChangeInFairValueOfPIPESWarrantLiability" xlink:label="loc_NITOChangeInFairValueOfPIPESWarrantLiability" />
      <link:calculationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOChangeInFairValueOfPIPESWarrantLiability" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_GainLossOnInvestment" xlink:label="loc_NITOGainLossOnInvestment" />
      <link:calculationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOGainLossOnInvestment" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInLongTermReceivablesCurrent" xlink:label="loc_us-gaapIncreaseDecreaseInLongTermReceivablesCurrent" />
      <link:calculationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInLongTermReceivablesCurrent" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_IncreaseDecreaseInInterestIncomeFromSolarProject" xlink:label="loc_NITOIncreaseDecreaseInInterestIncomeFromSolarProject" />
      <link:calculationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOIncreaseDecreaseInInterestIncomeFromSolarProject" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInMarketableSecuritiesRestricted" xlink:label="loc_us-gaapIncreaseDecreaseInMarketableSecuritiesRestricted" />
      <link:calculationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInMarketableSecuritiesRestricted" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_IncreaseDecreaseInExchangeRateDifferencesOnOperatingLeases" xlink:label="loc_NITOIncreaseDecreaseInExchangeRateDifferencesOnOperatingLeases" />
      <link:calculationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOIncreaseDecreaseInExchangeRateDifferencesOnOperatingLeases" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable" xlink:label="loc_us-gaapIncreaseDecreaseInAccountsReceivable" />
      <link:calculationArc order="20" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInAccountsReceivable" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInInventories" xlink:label="loc_us-gaapIncreaseDecreaseInInventories" />
      <link:calculationArc order="21" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInInventories" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:label="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" />
      <link:calculationArc order="22" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInAccountsPayable" xlink:label="loc_us-gaapIncreaseDecreaseInAccountsPayable" />
      <link:calculationArc order="23" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInAccountsPayable" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInOtherOperatingLiabilities" xlink:label="loc_us-gaapIncreaseDecreaseInOtherOperatingLiabilities" />
      <link:calculationArc order="24" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInOtherOperatingLiabilities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_IncreaseDecreaseInOperatingLeaseExpense" xlink:label="loc_NITOIncreaseDecreaseInOperatingLeaseExpense" />
      <link:calculationArc order="25" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOIncreaseDecreaseInOperatingLeaseExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_IncreaseDecreaseInOperatingLeaseAssets" xlink:label="loc_NITOIncreaseDecreaseInOperatingLeaseAssets" />
      <link:calculationArc order="26" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_NITOIncreaseDecreaseInOperatingLeaseAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability" xlink:label="loc_us-gaapIncreaseDecreaseInOperatingLeaseLiability" />
      <link:calculationArc order="27" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInOperatingLeaseLiability" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PaymentsToAcquireOtherInvestments" xlink:label="loc_us-gaapPaymentsToAcquireOtherInvestments" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToAcquireOtherInvestments" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" xlink:label="loc_us-gaapProceedsFromSaleOfPropertyPlantAndEquipment" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapProceedsFromSaleOfPropertyPlantAndEquipment" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PaymentsToFundLongtermLoansToRelatedParties" xlink:label="loc_us-gaapPaymentsToFundLongtermLoansToRelatedParties" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToFundLongtermLoansToRelatedParties" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PaymentsToAcquireMarketableSecurities" xlink:label="loc_us-gaapPaymentsToAcquireMarketableSecurities" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToAcquireMarketableSecurities" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PaymentsForProceedsFromLoansReceivable" xlink:label="loc_us-gaapPaymentsForProceedsFromLoansReceivable" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsForProceedsFromLoansReceivable" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_PaymentsToInvestmentInBatteryStorageProjects" xlink:label="loc_NITOPaymentsToInvestmentInBatteryStorageProjects" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_NITOPaymentsToInvestmentInBatteryStorageProjects" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_PaymentsOnLoansGantedOrSolarPVJointVentureProject" xlink:label="loc_NITOPaymentsOnLoansGantedOrSolarPVJointVentureProject" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_NITOPaymentsOnLoansGantedOrSolarPVJointVentureProject" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ProceedsFromSaleOfSubsidiaryNetOfCashDisposed" xlink:label="loc_NITOProceedsFromSaleOfSubsidiaryNetOfCashDisposed" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_NITOProceedsFromSaleOfSubsidiaryNetOfCashDisposed" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromNotesPayable" xlink:label="loc_us-gaapProceedsFromNotesPayable" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapProceedsFromNotesPayable" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RepaymentsOfNotesPayable" xlink:label="loc_us-gaapRepaymentsOfNotesPayable" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapRepaymentsOfNotesPayable" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromLinesOfCredit" xlink:label="loc_us-gaapProceedsFromLinesOfCredit" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapProceedsFromLinesOfCredit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RepaymentsOfLinesOfCredit" xlink:label="loc_us-gaapRepaymentsOfLinesOfCredit" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapRepaymentsOfLinesOfCredit" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ProceedsFromPIPEAgreement" xlink:label="loc_NITOProceedsFromPIPEAgreement" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_NITOProceedsFromPIPEAgreement" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromWarrantExercises" xlink:label="loc_us-gaapProceedsFromWarrantExercises" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapProceedsFromWarrantExercises" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_ProceedsFromStandbyEquityPurchaseAgreementNet" xlink:label="loc_NITOProceedsFromStandbyEquityPurchaseAgreementNet" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_NITOProceedsFromStandbyEquityPurchaseAgreementNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:label="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:type="arc" weight="1" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" xlink:label="loc_us-gaapEffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapEffectOfExchangeRateOnCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/General" xlink:title="999007 - Disclosure - GENERAL" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentation" xlink:title="999008 - Disclosure - SUMMARY OF SIGNIFICANT ACCOUNTING POLICIES AND BASIS OF PRESENTATION" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/OtherCurrentAssets" xlink:title="999009 - Disclosure - OTHER CURRENT ASSETS" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/MitocarexTransaction" xlink:title="999010 - Disclosure - MITOCAREX TRANSACTION" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/PropertyAndEquipmentNet" xlink:title="999011 - Disclosure - PROPERTY AND EQUIPMENT, NET" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InvestmentInNonconsolidatedAffiliate" xlink:title="999012 - Disclosure - INVESTMENT IN NONCONSOLIDATED AFFILIATE" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InvestmentAndLoanToSolterra" xlink:title="999013 - Disclosure - INVESTMENT AND LOAN TO SOLTERRA" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SolarPhotovolaicJointVentureProject" xlink:title="999014 - Disclosure - SOLAR PHOTOVOLAIC JOINT VENTURE PROJECT" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ConsolidatedEntity" xlink:title="999015 - Disclosure - A CONSOLIDATED ENTITY" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/OtherLiabilities" xlink:title="999016 - Disclosure - OTHER LIABILITIES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CommitmentAndContingentLiabilities" xlink:title="999017 - Disclosure - COMMITMENT AND CONTINGENT LIABILITIES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/Leases" xlink:title="999018 - Disclosure - LEASES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CommonStockAndWarrants" xlink:title="999019 - Disclosure - COMMON STOCK AND WARRANTS" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/StockOptions" xlink:title="999020 - Disclosure - STOCK OPTIONS" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CostOfSales" xlink:title="999021 - Disclosure - COST OF SALES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ResearchAndDevelopmentExpenses" xlink:title="999022 - Disclosure - RESEARCH AND DEVELOPMENT EXPENSES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SellingAndMarketingExpenses" xlink:title="999023 - Disclosure - SELLING AND MARKETING EXPENSES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/GeneralAndAdministrativeExpenses" xlink:title="999024 - Disclosure - GENERAL AND ADMINISTRATIVE EXPENSES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/FinancingExpensesIncomeNet" xlink:title="999025 - Disclosure - FINANCING EXPENSES (INCOME), NET" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/IncomeTax" xlink:title="999026 - Disclosure - INCOME TAX" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/LossPerShare" xlink:title="999027 - Disclosure - LOSS PER SHARE" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/RelatedParties" xlink:title="999028 - Disclosure - RELATED PARTIES" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SegmentReporting" xlink:title="999029 - Disclosure - SEGMENT REPORTING" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SubsequentEvents" xlink:title="999030 - Disclosure - SUBSEQUENT EVENTS" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CreditFacility" xlink:title="999031 - Disclosure - CREDIT FACILITY" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/DiscontinuedOperations" xlink:title="999032 - Disclosure - DISCONTINUED OPERATIONS" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationPolicies" xlink:title="999033 - Disclosure - SUMMARY OF SIGNIFICANT ACCOUNTING POLICIES AND BASIS OF PRESENTATION (Policies)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationTables" xlink:title="999034 - Disclosure - SUMMARY OF SIGNIFICANT ACCOUNTING POLICIES AND BASIS OF PRESENTATION (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/OtherCurrentAssetsTables" xlink:title="999035 - Disclosure - OTHER CURRENT ASSETS (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/PropertyAndEquipmentNetTables" xlink:title="999036 - Disclosure - PROPERTY AND EQUIPMENT, NET (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InvestmentInNonconsolidatedAffiliateTables" xlink:title="999037 - Disclosure - INVESTMENT IN NONCONSOLIDATED AFFILIATE (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/OtherLiabilitiesTables" xlink:title="999038 - Disclosure - OTHER LIABILITIES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CommitmentAndContingentLiabilitiesTables" xlink:title="999039 - Disclosure - COMMITMENT AND CONTINGENT LIABILITIES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/LeasesTables" xlink:title="999040 - Disclosure - LEASES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/StockOptionsTables" xlink:title="999041 - Disclosure - STOCK OPTIONS (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CostOfSalesTables" xlink:title="999042 - Disclosure - COST OF SALES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ResearchAndDevelopmentExpensesTables" xlink:title="999043 - Disclosure - RESEARCH AND DEVELOPMENT EXPENSES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SellingAndMarketingExpensesTables" xlink:title="999044 - Disclosure - SELLING AND MARKETING EXPENSES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/GeneralAndAdministrativeExpensesTables" xlink:title="999045 - Disclosure - GENERAL AND ADMINISTRATIVE EXPENSES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/FinancingExpensesIncomeNetTables" xlink:title="999046 - Disclosure - FINANCING EXPENSES (INCOME), NET (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/IncomeTaxTables" xlink:title="999047 - Disclosure - INCOME TAX (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/LossPerShareTables" xlink:title="999048 - Disclosure - LOSS PER SHARE (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/RelatedPartiesTables" xlink:title="999049 - Disclosure - RELATED PARTIES (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SegmentReportingTables" xlink:title="999050 - Disclosure - SEGMENT REPORTING (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CreditFacilityTables" xlink:title="999051 - Disclosure - CREDIT FACILITY (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/DiscontinuedOperationsTables" xlink:title="999052 - Disclosure - DISCONTINUED OPERATIONS (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/GeneralDetailsNarrative" xlink:title="999053 - Disclosure - GENERAL (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfPropertyPlantAndEquipmentDepreciationRatesDetails" xlink:title="999054 - Disclosure - SCHEDULE OF PROPERTY, PLANT AND EQUIPMENT DEPRECIATION RATES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfFairValueAssetsOnRecurringBasisDetails" xlink:title="999055 - Disclosure - SCHEDULE OF FAIR VALUE ASSETS ON RECURRING BASIS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfChangesInFairValueOfAssetsDetails" xlink:title="999056 - Disclosure - SCHEDULE OF CHANGES IN FAIR VALUE OF ASSETS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SummaryOfSignificantAccountingPoliciesAndBasisOfPresentationDetailsNarrative" xlink:title="999057 - Disclosure - SUMMARY OF SIGNIFICANT ACCOUNTING POLICIES AND BASIS OF PRESENTATION (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfOtherCurrentAssetsDetails" xlink:title="999058 - Disclosure - SCHEDULE OF OTHER CURRENT ASSETS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/MitocarexTransactionDetailsNarrative" xlink:title="999059 - Disclosure - MITOCAREX TRANSACTION (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfPropertyPlantAndEquipmentDetails" xlink:title="999060 - Disclosure - SCHEDULE OF PROPERTY PLANT AND EQUIPMENT (Details)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaapPropertyPlantAndEquipmentNet" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PropertyPlantAndEquipmentGross" xlink:label="loc_us-gaapPropertyPlantAndEquipmentGross" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapPropertyPlantAndEquipmentNet" xlink:to="loc_us-gaapPropertyPlantAndEquipmentGross" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:label="loc_us-gaapAccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapPropertyPlantAndEquipmentNet" xlink:to="loc_us-gaapAccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:type="arc" weight="-1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/PropertyAndEquipmentNetDetailsNarrative" xlink:title="999061 - Disclosure - PROPERTY AND EQUIPMENT, NET (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfFairValueConversionDetails" xlink:title="999062 - Disclosure - SCHEDULE OF FAIR VALUE CONVERSION (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfEquityInvestmentDetails" xlink:title="999063 - Disclosure - SCHEDULE OF EQUITY INVESTMENT (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InvestmentInNonconsolidatedAffiliateDetailsNarrative" xlink:title="999064 - Disclosure - INVESTMENT IN NONCONSOLIDATED AFFILIATE (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/InvestmentAndLoanToSolterraDetailsNarrative" xlink:title="999065 - Disclosure - INVESTMENT AND LOAN TO SOLTERRA (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SolarPhotovolaicJointVentureProjectDetailsNarrative" xlink:title="999066 - Disclosure - SOLAR PHOTOVOLAIC JOINT VENTURE PROJECT (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ConsolidatedEntityDetailsNarrative" xlink:title="999067 - Disclosure - A CONSOLIDATED ENTITY (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfOtherLiabilitiesDetails" xlink:title="999068 - Disclosure - SCHEDULE OF OTHER LIABILITIES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfWarrantAssumptionsUsedDetails" xlink:title="999069 - Disclosure - SCHEDULE OF WARRANT ASSUMPTIONS USED (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CommitmentAndContingentLiabilitiesDetailsNarrative" xlink:title="999070 - Disclosure - COMMITMENT AND CONTINGENT LIABILITIES (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfOperatingLeaseCostDetails" xlink:title="999071 - Disclosure - SCHEDULE OF OPERATING LEASE COST (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfSupplementalCashFlowInformationRelatedToOperatingLeaseDetails" xlink:title="999072 - Disclosure - SCHEDULE OF SUPPLEMENTAL CASH FLOW INFORMATION RELATED TO OPERATING LEASE (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfSupplementalBalanceSheetInformationRelatedToOperatingLeasesDetails" xlink:title="999073 - Disclosure - SCHEDULE OF SUPPLEMENTAL BALANCE SHEET INFORMATION RELATED TO OPERATING LEASES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfMinimumLeasePaymentsUnderNon-CancelableLeasesDetails" xlink:title="999074 - Disclosure - SCHEDULE OF MINIMUM LEASE PAYMENTS UNDER NON- CANCELABLE LEASES (Details)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue" xlink:label="loc_us-gaapLesseeOperatingLeaseLiabilityPaymentsDue" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextRollingTwelveMonths" xlink:label="loc_us-gaapLesseeOperatingLeaseLiabilityPaymentsDueNextRollingTwelveMonths" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLesseeOperatingLeaseLiabilityPaymentsDue" xlink:to="loc_us-gaapLesseeOperatingLeaseLiabilityPaymentsDueNextRollingTwelveMonths" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/LeasesDetailsNarrative" xlink:title="999075 - Disclosure - LEASES (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetailsParenthetical" xlink:title="999076 - Disclosure - SCHEDULE OF TRANSACTIONS AND BALANCES WITH RELATED PARTIES (Details) (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CommonStockAndWarrantsDetailsNarrative" xlink:title="999077 - Disclosure - COMMON STOCK AND WARRANTS (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfEstimatedFairValueOfOptionsGrantedDetails" xlink:title="999078 - Disclosure - SCHEDULE OF ESTIMATED FAIR VALUE OF OPTIONS GRANTED (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfEstimatedFairValueOfOptionsGrantedDetailsParenthetical" xlink:title="999079 - Disclosure - SCHEDULE OF ESTIMATED FAIR VALUE OF OPTIONS GRANTED (Details) (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfStockOptionActivityDetails" xlink:title="999080 - Disclosure - SCHEDULE OF STOCK OPTION ACTIVITY (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/StockOptionsDetailsNarrative" xlink:title="999081 - Disclosure - STOCK OPTIONS (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfCostOfSalesDetails" xlink:title="999082 - Disclosure - SCHEDULE OF COST OF SALES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfResearchAndDevelopmentExpensesDetails" xlink:title="999083 - Disclosure - SCHEDULE OF RESEARCH AND DEVELOPMENT EXPENSES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfSellingAndMarketingExpensesDetails" xlink:title="999084 - Disclosure - SCHEDULE OF SELLING AND MARKETING EXPENSES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfGeneralAndAdministrativeExpensesDetails" xlink:title="999085 - Disclosure - SCHEDULE OF GENERAL AND ADMINISTRATIVE EXPENSES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfFinancingExpensesNetDetails" xlink:title="999086 - Disclosure - SCHEDULE OF FINANCING EXPENSES NET (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfIncomeTaxExpensesDetails" xlink:title="999087 - Disclosure - SCHEDULE OF INCOME TAX EXPENSES (Details)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxExpenseBenefit" xlink:label="loc_us-gaapIncomeTaxExpenseBenefit" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationMinorityInterestIncomeExpense" xlink:label="loc_us-gaapIncomeTaxReconciliationMinorityInterestIncomeExpense" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationMinorityInterestIncomeExpense" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate" xlink:label="loc_us-gaapEffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapEffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate" xlink:label="loc_us-gaapIncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationIncomeTaxExpenseBenefitAtFederalStatutoryIncomeTaxRate" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationNondeductibleExpense" xlink:label="loc_us-gaapIncomeTaxReconciliationNondeductibleExpense" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationNondeductibleExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationShareBasedCompensationExcessTaxBenefitAmount" xlink:label="loc_us-gaapEffectiveIncomeTaxRateReconciliationShareBasedCompensationExcessTaxBenefitAmount" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapEffectiveIncomeTaxRateReconciliationShareBasedCompensationExcessTaxBenefitAmount" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationDeductions" xlink:label="loc_us-gaapIncomeTaxReconciliationDeductions" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationDeductions" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationChangeInEnactedTaxRate" xlink:label="loc_us-gaapIncomeTaxReconciliationChangeInEnactedTaxRate" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationChangeInEnactedTaxRate" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance" xlink:label="loc_us-gaapIncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapIncomeTaxExpenseBenefit" xlink:to="loc_us-gaapIncomeTaxReconciliationChangeInDeferredTaxAssetsValuationAllowance" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfDeferredTaxAssetsAndLiabilitiesDetails" xlink:title="999088 - Disclosure - SCHEDULE OF DEFERRED TAX ASSETS AND LIABILITIES (Details)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsGross" xlink:label="loc_us-gaapDeferredTaxAssetsGross" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsEmployeeCompensation" xlink:label="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsEmployeeCompensation" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsEmployeeCompensation" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsOtherLossCarryforwards" xlink:label="loc_us-gaapDeferredTaxAssetsOtherLossCarryforwards" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsOtherLossCarryforwards" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="nito-20250630.xsd#NITO_DeferredTaxOperatingLeaseLiabilities" xlink:label="loc_NITODeferredTaxOperatingLeaseLiabilities" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_NITODeferredTaxOperatingLeaseLiabilities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost" xlink:label="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsOther" xlink:label="loc_us-gaapDeferredTaxAssetsOther" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsGross" xlink:to="loc_us-gaapDeferredTaxAssetsOther" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredIncomeTaxLiabilities" xlink:label="loc_us-gaapDeferredIncomeTaxLiabilities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxLiabilitiesLeasingArrangements" xlink:label="loc_us-gaapDeferredTaxLiabilitiesLeasingArrangements" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredIncomeTaxLiabilities" xlink:to="loc_us-gaapDeferredTaxLiabilitiesLeasingArrangements" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DeferredTaxAssetsNet" xlink:label="loc_us-gaapDeferredTaxAssetsNet" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsNet" xlink:to="loc_us-gaapDeferredTaxAssetsGross" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapDeferredTaxAssetsNet" xlink:to="loc_us-gaapDeferredIncomeTaxLiabilities" xlink:type="arc" weight="-1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/IncomeTaxDetailsNarrative" xlink:title="999089 - Disclosure - INCOME TAX (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfBasicAndDilutedLossPerShareDetails" xlink:title="999090 - Disclosure - SCHEDULE OF BASIC AND DILUTED LOSS PER SHARE (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfTransactionsAndBalancesWithRelatedPartiesDetails" xlink:title="999091 - Disclosure - SCHEDULE OF TRANSACTIONS AND BALANCES WITH RELATED PARTIES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/RelatedPartiesDetailsNarrative" xlink:title="999092 - Disclosure - RELATED PARTIES (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfInformationRelatedToOperationsOfReportableOperatingSegmentsDetails" xlink:title="999093 - Disclosure - SCHEDULE OF INFORMATION RELATED TO OPERATIONS OF REPORTABLE OPERATING SEGMENTS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfInformationOnSalesByGeographicDistributionDetails" xlink:title="999094 - Disclosure - SCHEDULE OF INFORMATION ON SALES BY GEOGRAPHIC DISTRIBUTION (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfSalesToCustomersDetails" xlink:title="999095 - Disclosure - SCHEDULE OF SALES TO CUSTOMERS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfInformationOnLongLivedAssetsDetails" xlink:title="999096 - Disclosure - SCHEDULE OF INFORMATION ON LONG LIVED ASSETS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SegmentReportingDetailsNarrative" xlink:title="999097 - Disclosure - SEGMENT REPORTING (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfFairValueOfLevels3AssetsDetails" xlink:title="999098 - Disclosure - SCHEDULE OF FAIR VALUE OF THE LEVELS 3 ASSETS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/SubsequentEventsDetailsNarrative" xlink:title="999099 - Disclosure - SUBSEQUENT EVENTS (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfEstimatedFairValueOfWarrantSharesDetails" xlink:title="999100 - Disclosure - SCHEDULE OF ESTIMATED FAIR VALUE OF WARRANT SHARES (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/CreditFacilityDetailsNarrative" xlink:title="999101 - Disclosure - CREDIT FACILITY (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/ScheduleOfEstimatedFairValueOfWarrantSharesAssumptionsDetails" xlink:title="999102 - Disclosure - SCHEDULE OF ESTIMATED FAIR VALUE OF WARRANT SHARES ASSUMPTIONS (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://n2off.com/role/DiscontinuedOperationsDetailsNarrative" xlink:title="999103 - Disclosure - DISCONTINUED OPERATIONS (Details Narrative)" />
</link:linkbase>
